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. 2019 Dec 5;10(12):1012. doi: 10.3390/genes10121012

Figure 3.

Figure 3

Subcellular localization of GmFWL1-GFP in soybean root hairs after treatment with L-kynurenine, an auxin biosynthesis inhibitor (A–C), or after a treatment with L-kynurenine and 2,3,5-triiodobenzoic acid, an inhibitor of auxin transport (D,E). Twenty-four hours after treatment, the plants were mock-inoculated (A) or inoculated with B. diazoefficiens (B,D). Soybean root hair cells expressing the GmFWL1-GFP chimeric protein were observed 24 h after inoculation under an epifluorescent confocal microscope. The chimeric protein accumulates at the tip of the root hair in response to B. diazoefficiens only upon L-kynurenine treatment (white arrows). (C,E): Quantification of the intensity of the GmFWL1-GFP signal (y-axis) across transgenic soybean root hair cells (x-axis, μm) in inoculated (Lykn-1DAI) and mock-inoculated L-kynurenine-treated (Lykn) transgenic root hair cells (C), and in inoculated L-kynurenine/2,3,5-triiodobenzoic acid transgenic root hair cells (E; the arrow highlight the position of the root hair tip). Scale = 10 μm. The pictures shown are representative from a series of observations conducted on three independent biological experiments.