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. 2019 Nov 25;10(12):968. doi: 10.3390/genes10120968

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RdRp derivatives incapable of recombination in cells exhibit defects in copy-choice recombination (template switching) in vitro. (A) Products of reactions using oligo/poly(rA) as template reflect copy-choice recombination, which requires the template-switching activity of the RdRp [32]. RdRp engages primed template and initiates RNA synthesis. The RdRp-nascent RNA complex moves to new template from internal positions or as a consequence of reaching the end of template, thus creating greater-than-template-length products. (B) Specific poly (rU) polymerase activity (pmol/min/µg) of each RdRp. WT and the indicated PV RdRp variants assessed by using a poly (rU) polymerase activity assay. Error bars represent SEM (n = 3). Statistical analyses were performed using unpaired, two-tailed t-test (** significance level p ≤ 0.01, n.s. = non-significant).