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. 2019 Nov 21;10(12):958. doi: 10.3390/genes10120958

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of RNA-Seq data using qPCR. (a) Consistency of log₂FoldChange (log₂FC) between RNA-Seq (X-axis) and qPCR assay (Y-axis) is high (R² = 0.814) based on the 15 selected genes. (b) Log₂FC of the selected DEGs by qPCR compared with RNA-Seq data. The log₂FC values of qPCR are shown as the means ± standard error (SE).