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. 2019 Dec 12;10(12):1037. doi: 10.3390/genes10121037

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Indirect immunostaining of insulator proteins CHROMATOR (A) and dCTC-Factor (B) in the 3C region of the polytene X chromosome. DNA staining (black), CHROMATOR (CHRO) and CTCF signals (green). Red arrows point to the thin band 3C7, containing the body of the Notch gene. The top in each panel features the cytological map of 3C region according to Bridges (1938), lines connect some of the marker bands. Yellow and blue arrows show an overlay of CHRO and dCTCF immunostaining signals in 3C6/C7 interband, respectively. Yellow and blue arrowheads show CHRO and dCTCF signals adjacent to the above signals and used as a reference. O-R: wild-type line; fa-[swb]:N^fa-swb deletion. Bar, 5 µm.