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. 2019 Nov 28;10(12):981. doi: 10.3390/genes10120981

Figure 3.

Figure 3

Construction of fragment-by-fragment deletion vector of FAM13A gene promoter and identification gel electrophoresis map of 5 recombinant vectors constructed by enzyme activity assay (A) −898/+54, −659/+54, −512/+54, −241+54, and −79/+54, where lane 1 is PGL3-basic empty vector and lanes 2–6 are different fragment-by-fragment deletions. (B) After transfecting recombinant PGL3-basic vector into bovine precursor adipocytes for 48 hours, double luciferase activity was measured and analyzed statistically (* indicates P < 0.05, ** indicates P < 0.01).