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. 2019 Nov 28;10(12):981. doi: 10.3390/genes10120981

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Electrophoretic mobility shift assay (EMSA) transcription factors ACSL1 and ASCL2. (A) EMSA test verifies binding of transcription factor ACSL1. (B) EMSA test verifies binding of transcription factor ASCL2. Only biotin-labeled probes are added to lane 1, biotin-labeled probes and proteins are added to lane 2, labeled competitive probes are added to lane 3, mutation probes are added to lane 4, and antibodies corresponding to transcription factors are added to lane 5.