Table 3.
Biotin-labeled probe, competitive probe, and mutation probe information of key transcription factors in the FAM13A gene promoter region.
| Primer | Primer Sequence (5′–3′) | Annealing Temperature (°C) |
|---|---|---|
| ACSL1-bio-F | Biotin-TGGCTGGGTGGTTCAGCTGCTTCCCTGGAACAGA | 75 |
| ACSL1-bio-R | Biotin-TCTGTTCCAGGGAAGCAGCTGAACCACCCAGCCA | 75 |
| ACSL1-jz-F | TGGCTGGGTGGTTCAGCTGCTTCCCTGGAACAGA | 75 |
| ACSL1-jz-R | TCTGTTCCAGGGAAGCAGCTGAACCACCCAGCCA | 75 |
| ACSL1-mut-F | TGGCTGGGTGGTTCAGACCCTTCCCTGGAACAGA | 75 |
| ASCL2-mut-R | TCTGTTCCAGGGAAGGGTCTGAACCACCCAGCCA | 75 |
| ASCL2-bio-F | Biotin-TAAGCCCTCCAGCAGCTGCTCCTCTGATTGGCT | 74 |
| ASCL2-bio-R | Biotin-AGCCAATCAGAGGAGCAGCTGCTGGAGGGCTTA | 74 |
| ASCL2-jz-F | TAAGCCCTCCAGCAGCTGCTCCTCTGATTGGCT | 74 |
| ASCL2-jz-R | AGCCAATCAGAGGAGCAGCTGCTGGAGGGCTTA | 74 |
| ASCL2-mut-F | TAAGCCCTCCAGCAGGCCCTCCTCTGATTGGCT | 75 |
| ASCL2-mut-R | AGCCAATCAGAGGAGGGCCTGCTGGAGGGCTTA | 75 |