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. 2019 Dec 12;10(12):1038. doi: 10.3390/genes10121038

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Undifferentiated bovine granulosa cells were isolated from 3 to 8 mm follicles and cultured with or without FSH (0, 1, 5, 10, 25, and 50 ng/mL). The transcript abundance of AMH (A) AMHR II (B) BMP2 (C) BMP6 (D) LHCGR (E), and FSHR (F) genes in cultured granulosa cells. Data were standardized according to the GAPDH reference gene and expressed as fold difference ± standard error of mean compared with untreated control. Means without common letters are significantly different (p < 0.05; n = 5 independent cultures). FSHR = Follicle Stimulating Hormone Receptor. LHCGR = Luteinizing Hormone/Choriogonadotropin Receptor. AMH = Anti-Mullerian Hormone. AMHR-I I = Anti-Mullerian Hormone Receptor type-II. BMP2 = Bone Morphogenetic Protein 2. BMP6 = Bone Morphogenetic Protein 6.