Table 1.
Primers used in this study.
| Primers | Sequence | Tm (°C) | Size (bp) |
|---|---|---|---|
| TNXB-LongPCR-F | GTCTCTGCCCTGGGAATGA | 60 | 4900 |
| TNXB-LongPCR-R | TGTAAACACAGTGCTGCGA | ||
| TNXB frag1 For | GGCCAAGCCTGGAAGATAAA | 60 | 662 |
| TNXB frag1 Rev | GATTGGAGACAGAAGCACAC | ||
| TNXB frag2 For | CCAGGGAGAGAGGATGGAT | 60 | 671 |
| TNXB frag2 Rev | GTCCCCAGGAATGGAAGT | ||
| TNXB frag3 For | GACCTAGTGCCTCAGCCA | 60 | 733 |
| TNXB frag3 Rev | GGCTCTCTCTACTCCGTG | ||
| TNXB frag4 For | ATGGGTGGGAGTTGAGAG | 60 | 727 |
| TNXB frag4 Rev | TGGAAGCTGAGCAGGTAG | ||
| TNXB frag5 For | TCTCCTCTTCCTGCTTTCCC | 60 | 643 |
| TNXB frag5 Rev | CCCCATCAGTCTCCATGTC | ||
| TNXB frag6 For | CAGGACCAGCACCATCTT | 60 | 741 |
| TNXB frag6 Rev | TTGAGGTTGGCGTAGTGG | ||
| TNXB frag7 For | GCTGTCTCCTACCGAGGG | 60 | 621 |
| TNXB frag7 Rev | GCAGAGAAGGCTTCCTCC | ||
| TNXB_EX3Fw | GGTTGCAGTCAGAGGGGGCG | 58 | 300 |
| TNXB_EX3Rv | GCCCGCGACCTCTACAGTCG | ||
| TNXB_EX35Fw | GGGAGCCTCAGAGTGTGC | 58 | 480 |
| TNXB_EX35Rv | TCAGCCCCTGGAGTTTCTG |