Figure 2.

Noninvasive synovitis assessment in mice with CAIA. a) In vivo imaging of mouse forepaws after the intravenous injection of Dex700 (25 nmol, red color) and ICG (50 nmol, green color) 30 min prior to imaging. Scale bars = 1 cm. Images are representative of independent experiments (n = 3). Arrows indicate the clearance of each NIR fluorophore from the bloodstream. b,c) Quantitative scoring (SBR) charts of Dex700 and ICG for D180 in the inflammatory stages. SBR was calculated by the fluorescence intensity of the forepaw versus the background (bg) signal of the neighboring blank area (n = 3, mean ± s.d.; *P < 0.05, **P < 0.01, and ***P < 0.001). d) Clinical arthritis scores in the paws. The total score per mouse is 0–16.