Figure 2.

Specific deletion of Ddb1 in Sertoli cells. (A) The schematic diagram showing the generation of Ddb1 cKO mice; (B) immunostaining of DDB1 in control and cKO testicular sections, Arrowheads indicate DDB1 positive Sertoli cells and arrows indicate DDB1 negative Sertoli cells, scale bars = 50 μm; (C) Western blotting analysis of DDB1 in isolated Sertoli and germ cells from 14 dpp control and cKO mice, GAPDH was used as loading control; (D) quantitative results of Western blotting experiments as shown in (C). The DDB1 protein level was normalized against GAPDH. Control group data were set as 1. Data were presented as mean ± S.E.M. ** p < 0.01, Student’s t-test.