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. 2019 Nov 26;21(2):607–614. doi: 10.3892/mmr.2019.10851

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — MV induces edema and an inflammatory response in the lungs of rats. After the rats were treated with MV (6 ml/kg or 20 ml/kg), pulmonary edema was determined by (A) the wet/dry weight ratio and (B) the protein levels in BALF. (C) Lung MPO activity was also assessed. The mRNA levels of (D) ICAM-1, (E) VCAM-1, (F) TNF-α and (G) IL-8 were assessed. (H) The protein expression of VCAM-1, ICAM-1, TNF-α and IL-8 were assessed. Blots are representative of at least three independent experiments. (I) Semi-quantification of immunoblots shown in (H). The data are presented as the means ± SD (n=8). *P<0.05, **P<0.01, compared with the Control group. MV, mechanical ventilation; LV, ventilated with a low tidal volume; HV, ventilated with a high tidal volume.