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. 2019 Nov 29;21(2):705–719. doi: 10.3892/mmr.2019.10856

Figure 5.

Figure 5.

Binding of miR-337-3p to the 3′-UTR of ARHGAP10 and downregulation of ARHGAP10 expression by miR-337-3p in metastatic gastric tumor cells. (A) Schematic illustration revealing the potential binding sites of miR-337-3p at the 3′-UTR of ARHGAP10. miR-337-3p seed sequences and their complementary sequences on 3′-UTR are highlighted in blue. (B) pMIR-REPORT vectors containing the 3′-UTR of ARHGAP10 and control, miR-337-3p mimic, control inhibitor, or miR-337-3p inhibitor were co-transfected into 293T cells. At 48 h after transfection, the cells were lysed and the dual-luciferase reporter assay system was used to detect luciferase activity. After transfection of SGC-7901 cells with control and miR-337-3p mimic, the cells were subjected to (C) ARHGAP10 mRNA expression analysis by reverse transcription-quantitative PCR and (D) ARHGAP10 protein expression analysis by western blotting. The data are expressed as the mean ± SD of three independent transfection experiments. *P<0.05 and **P<0.01. ARH, ARHGAP10; miR, microRNA; NC, negative control; UTR, untranslated region.