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. 2020 Jan 7;10:4. doi: 10.1186/s13578-019-0371-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of SNHG6 alleviated ox-LDL-induced injury of HUVECs. a The abundance of SNHG6 in HUVECs transfected with si-NC, si-SNHG6#1 or si-SNHG6#2 was examined by RT-qPCR. HUVECs were transfected with si-NC, si-SNHG6#1, or si-SNHG6#2 and then treated with or without 25 µg/ml ox-LDL for 24 h. b The expression of SNHG6 was detected by RT-qPCR assay. c Cell viability was determined by CCK-8 assay. d Caspase-3 activity was measured using commercially-available kit. e Cell apoptosis was assessed by flow cytometry. f The expression of apoptosis-related protein Bax and Bcl-2 was examined by western blot assay. g, h The release of inflammatory cytokines IL-6 and TNF-α were assessed by ELISA assay. i, j The production of oxidative status markers ROS and MDA were determined using commercially-available kits. *P < 0.05 and **P < 0.01; ^#P < 0.05 and ^##P < 0.01