Fig. 5.

ROCK1 and ROCK2 were identified as the targets of miR-135a-5p. a Potential sites targeted by miR-135a-5p in the 3′UTR of ROCK1 and ROCK2. b, c Wild-type or mutant ROCK1 or ROCK2 reporter were co-transfected into HEK293T cells with miR-135a-5p mimics or miR-NC mimics, followed by the detection of luciferase activity. d, e RIP assay was performed to examine the enrichment levels of ROCK1, ROCK2 and miR-135a-5p in IgG or Ago2 immunoprecipitation complex. f HUVECs were transfected with miR-135a-5p mimics, miR-135a-5p inhibitors, or relative control, and then ROCK1 and ROCK2 protein levels were detected by RT-qPCR. g, h, j, k The abundance of ROCK1 and ROCK2 in ox-LDL-induced HUVECs and the serum of atherosclerosis patients were determined by RT-qPCR. i, l The inverse correlation between miR-135a-5p and ROCK1 or ROCK2 expression in atherosclerosis samples. *P < 0.05 and **P < 0.01; ^##P < 0.01