Figure 4: Gelatin zymography of tendon shows significant increase of proteases following injury.
(A-B) cathepsin (pH=4 buffer) zymography gel and densitometry quantification (C-D) cathepsin (pH=6 buffer) zymography gel and densitometry quantification (E-F) MMP zymography gel and densitometry quantification. Recombinant protein used as standard: cathepsin L, cathepsin K, MMP-2 respectively. *p<0.05. N=6-8.