Figure 3. NMDAR function is impaired in neurons from ASD subjects with PTCHD1-AS microdeletions.

(A) Representative traces displaying NMDA-evoked currents recorded at membrane potentials of −60 mV in iPSC-derived neurons (8 week-old) in the absence of extracellular Mg2+ (left). A plot showing current-voltage relationship of NMDA-evoked currents in the three neurons displayed in the left panel, recorded in the absence of extracellular Mg2+ (right).
(B) Scatter plots displaying all data points for NMDA-evoked currents recorded at −60 mV in the absence of extracellular Mg2+. Graphs also display mean and SEM. N=neurons/iPSC lines. *P < 0.05; ***P < 0.001, one-way ANOVA with Dunnett’s posthoc test.
(C) Representative traces of NMDA-evoked currents from 8 week-old iPSC-derived neurons, recorded at membrane potentials from −60 mV to +60 mV in the presence of extracellular Mg2+ (1 mM).
(D) Current-voltage relationship of NMDA-evoked currents recorded in the presence of extracellular Mg2+ at 1 mM. Graphs display mean and SEM. N=neurons/iPSC lines. *P < 0.05; **P < 0.01, one-way ANOVA with Dunnett’s posthoc test, compared at +60 mV.