Fig. 5.

PLP2 is involved in the biological pathogenesis of ccRCC. (A-D) PLP2 is upregulated in ccRCC tissues and renal cancer cells, as assessed by qRT-PCR and western blotting analysis. (E) Gene set enrichment analysis (GSEA) was used to compare high and low PLP2 expression groups in the TCGA database. Enrichment curves are shown for activated gene sets related to EMT and the G2M checkpoint. (F) Silencing of PLP2 with small RNA interference technology (si-PLP2-1 and si-PLP2-2). (G) si-PLP2 significantly repressed the migration of A498 cells. (H) GSEA enrichment curves showed that low PLP2 expression was associated with fatty acid triacylglycerol metabolism, lipid catabolic processes and neutral lipid metabolic processes. (H) and (I) Silencing of PLP2 significantly promoted neutral lipid catabolic processes and eliminated abnormal lipid accumulation in A498 cancer cells. Original magnification 200x. Data indicate the means ± SEM. **P<0.01, *** P < 0.001 (t-test).