Table 2:
Mechanical and biochemical properties of MSC-laden constructs with various media formulations at 4 and 8 weeks (mean ± SD; n = 4/group).
CM+ | DEX- | ITS/BSA/LA- | Serum+ | |||||
---|---|---|---|---|---|---|---|---|
4w | 8w | 4w | 8w | 4w | 8w | 4w | 8w | |
EY(kPa) | 182 ± 12 | 480 ± 132 | 297 ± 23 | 211 ± 215 | 0 | 0 | 0 | 0 |
|G*| (kPa) | 2181 ± 181 | 3835 ± 597 | 3009 ± 193 | 2527 ± 1330 | 151 ± 80 | 674 ± 241 | 0 | 0 |
Diameter (mm) | 3.8 ± 0.1 | 4.1 ± 0.2 | 3.8 ± 0.2 | 3.9 ± 0.2 | 2.7 ± 0.2 | 2.4 ± 0.2 | 1 | 1 |
GAG (%WW) | 3.1 ± 0.3 | 4.7 ± 0.8 | 4.2 ± 0.4 | 3.3 ± 1.4 | 1.1 ± 0.4 | 0.8 ± 0.3 | 1.5 ± 0.8 | 0.4 ± 0.1 |
GAG (μg/const) | 756 ± 67 | 1543 ± 275 | 951 ± 156 | 804 ± 490 | 62 ± 11 | 47 ± 9 | 14 ± 2 | 15 ± 8 |
Collagen (%WW) | 1.7 ± 0.3 | 3.6 ± 1.9 | 2.5 ± 0.5 | 4.0 ± 1.1 | 1.7 ± 0.5 | 2.2 ± 0.8 | 1.3 ± 0.7 | 0.7 ± 0.2 |
Collagen (μg/const) | 414 ± 89 | 1243 ± 795 | 559 ± 69 | 926 ± 292 | 102 ± 11 | 135 ± 22 | 11 ± 4 | 27 ± 5 |