Figure 6.

Transfection of miR-28 Mimic Reduced Plk2 Expression and Cell Apoptosis/Death

(A) miR-128 expression. H9c2 cells were plated in a six-well plate 1 day prior to transfection. The cells were transfected with 1 μg miR-128 mimic, mimic control, miR-128 inhibitor, or inhibitor control with 2 μL Lipofectamine 2000. Twenty-four hours after transfection, total RNA was extracted, and miR-128 expression was detected by qRT-PCR using SYBR Green qPCR kits, normalized to control cells transfected with control mimic. n = 3; *p < 0.05. (B) Plk2 expression. Proteins were extracted from the above transfected cells 48 h after transfection. Plk2 expression was detected by western blotting with Plk2 antibodies. Left panel: representative images of western blotting; right panel: semiquantitative results of western blotting. n = 3; *p < 0.05. (C) mimR-128 reduced cell apoptosis. The above transfected cells were subjected to AA, and cell apoptosis was determined by Annexin V and PI staining and flow cytometry. Upper panel: representative image of flow cytometry; bottom panel: statistical results of flow cytometry. n = 3; *p < 0.05.