Figure 4.

The Radiotherapy Sensitization Effect of Mn-NMPs in Cancer Therapy

(A) Schematic of the radiotherapy sensitization effect of Mn-NMPs in cancer therapy.

(B) Oxygen generation with different treatment.

(C) HIF-1α expression level in B16-F10 cells with or without the treatment of Mn-NMPs. The cell nuclei and HIF-1α were stained with DAPI (blue) and anti-HIF-1α antibody (red), respectively.

(D) Cell viability of B16-F10 cells with different treatments in normoxia or hypoxia.

(E) Representative PA images of B16-F10 tumors with or without the treatment of Mn-NMPs.

(F) Immunofluorescence images of B16-F10 tumor after different treatments. The cell nuclei and HIF-1α were stained with DAPI (blue) and anti-HIF-1α antibody (red), respectively.

(G–I) Tumor volume changes (G), tumor weight (H), and the photograph of the tumors (I) with different treatments. Significance between each group was calculated using ANOVA with Tukey post hoc test. *p< 0.05, **p< 0.01, ***p< 0.001. Results are expressed as the mean ± S.D. of at least three independent experiments measured in triplicate.

See also Figures S6–S9.