Figure 2.

Overexpression of SFRP1 Suppressed Proliferation and Enhanced Apoptosis of RASFs

(A) The SFRP1 expression in RASFs and OASFs determined by qRT-PCR. GAPDH was used as an internal control. (B) SFRP1 expression in the synovial tissues of patients with RA and OA detected by immunohistochemical staining (×400). The synovial tissue sections of patients with RA were used as positive control. (C) Methylation of SFRP1 promoter region predicted through the bioinformatics website and identified by MSP assay (H₂O, double-negative control; IVD, methylation-positive control; NL, unmethylated positive control; U, unmethylation; M, methylation). (D) SFRP1 expression in Aza-dC-treated RASFs detected by qRT-PCR. (E–H) Proliferation, invasion (×200), migration, and apoptosis of RASFs were assessed upon overexpression of SFRP1 by means of WST-1 (E), Transwell assay (F), scratch test (G), and flow cytometry (H), respectively. **p < 0.05 compared with OASFs; *p < 0.05 compared with RASFs infected with lentivirus expressing oe-NC; ^#p < 0.05 compared with RASFs treated with DMSO. The results were expressed as mean ± SD. Comparisons between two groups were conducted by means of t test. The experiment was repeated three times.