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Primer Sequences for MSP and BSP
| Gene | Primer Sequence |
|---|---|
| SFRP1(M) | F, 5′-AGTTTGGTTAATATGGTGAAATTTC-3′ |
| R, 5′-ACCTAAACTAAAATACAATAACGCT-3′ | |
| SFRP1(U) | F, 5′-TTTGGTTAATATGGTGAAATTTTGT-3′ |
| R, 5′-TACCTAAACTAAAATACAATAACACT-3′ | |
| BSP | F, 5′-GTTTGGGAGGTTAAGGTAGGAGTAT-3′ |
| R, 5′-ACAAACCCATAAAATTATAAAAACTTTTT-3′ |
SFRP1, secreted frizzled-related protein 1; BSP, bisulfite sequencing PCR; F, forward; R, reverse.
