Fig. 7.

Inhibition of Cat K overcomes anti-cancer drugs resistance in vivo. (A–D) Mice bearing Caki xenograft tumors were treated with 5 mg/kg ODN, 3 mg/kg GST-TRAIL, ODN and GST-TRAIL or vehicle for 24 days. Tumor volume (A) and tumor size (B) were then measured. TUNEL assays were used to measure apoptosis in vivo (C). Protein expression was measured by western blotting (D). (E) Caki, mesangial cells (MC) and TCMK-1 cells were treated with 2 μM ODN, 50 ng/mL TRAIL, ODN plus TRAIL (E), or the indicated concentrations of ODN (F) for 24 h. Cell morphology was assessed using a microscope (E). Apoptosis (E) and protein expression (F) were measured by flow cytometry and western blotting, respectively. (positive control, p.c: Caki cell lysates). The values in the graph (A and E) represent the mean ± SD of three independent experiments. *p < 0.01 compared to the vehicle.