Figure 3.

miR-10b Overexpression Decreases hESC-CM Apoptosis

(A) Apoptosis of hESC-CMs transfected with NC mimics or miR-10b mimics followed by H₂O₂ treatment was analyzed by flow cytometry. The annexin V-APC (x axis)-positive area represents the early stage of apoptotic cells, and the PI (y axis)-positive area represents the late stage of apoptotic cells. (B) Percentage of total apoptotic hESC-CMs transfected with NC mimics or miR-10b mimics (n = 3). (C) Relative expression levels of genes related to apoptosis (BAX, DAPK1, STK17B) in hESC-CMs transfected with NC mimics or miR-10b mimics. GAPDH was used as an internal reference to calculate the relative mRNA levels (n = 3). Statistical significance was calculated using Student’s t test for paired samples. Data are shown as the means ± SEM. *p < 0.05, **p < 0.01.