Figure 4.

LATS1 is the Target of miR-10b

(A) Western blot analysis of LATS1 expression in hESC-CMs transfected with NC ormiR-10b mimics. (B) The predicted miR-10b binding site in the 3′ UTR of LATS1 mRNA (target 1). (C) Binding of miR-10b to the LATS1 3′ UTR was determined by a luciferase reporter assay (n = 3). (D) Biotin-labeled NC or miR-10b mimics were transfected into hESC-CMs. The 3′ UTR of LATS1 was pulled down by NC or miR-10b and quantified by qRT-PCR (n = 3). (E) Western blot analysis of LATS1 expression in hESC-CMs transfected with si-NC or si-LATS1. (F) Evaluation of hESC-CM proliferation after transfection with si-NC or si-LATS1 by EdU (green), α-actinin (red), and DAPI (blue) staining. (G) Percentage of EdU⁺ CMs transfected with si-NC or si-LATS1 (n = 3). Statistical significance was calculated using Student’s t test for paired samples. Data are shown as the means ± SEM. *p < 0.05, **p < 0.01.