Table 2.
Half inhibitory concentrations (IC50) of M. aquifolium extracts 1-5 on tumor and normal cell lines after 24-hour in vitro treatment, expressed as median value (μg/mL) ± standard error (SD) of logIC50. The median values were extracted from the dose-response sigmoid curve (logarithm of concentration versus growth inhibition percent, analysis performed with the GraphPad Prism 5 software) (see supplemental data ).
| Extract 1 | Extract 2 | Extract 3 | Extract 4 | Extract 5 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Median values | SD | Median values | SD | Median values | SD | Median values | SD | Median values | SD | |
| DLD-1 | 9.67 | 0.05 | 14.26 | 0.07 | 56.05∗ | 0.11 | 67.01∗ | 0.08 | 32.63 | 0.10 |
| A2780 | 12.72 | 0.06 | 22.59 | 0.08 | 72.23∗ | 0.11 | 57.16∗ | 0.09 | 52.59∗ | 0.12 |
| A375 | 15.76 | 0.05 | 19.85 | 0.06 | 70.89∗ | 0.05 | 26.86 | 0.08 | 36.42 | 0.06 |
| BJ | 11.03 | 0.04 | 25.06 | 0.05 | 53.55∗ | 0.15 | 102.81∗ | 0.22 | 73.01∗ | 0.11 |
The values above 50 μg/mL are hypothetical; they were obtained by extrapolation, given that the highest concentration used in the experiment was 50 μg/mL.