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. 2019 Dec 17;2019:9281481. doi: 10.1155/2019/9281481

Figure 1.

Figure 1

Effects of CUR on bone architecture properties of the femurs in iron-overload mice and formation of bone-mineralized nodules in osteoblastic MC3T3-E1 cells treated with excess iron. (a) Representative 3D reconstructed images analyzed by micro-CT. C57/BL6 male mice aged 2 months were treated with i.p. iron dextran (100 mg/kg) once a week for 3 months and simultaneously given NAC and various doses of CUR, and subsequently, bone architecture properties of the femur were analyzed by micro-CT. (b) Bone-mineralized nodules in osteoblastic MC3T3-E1 cells. Osteoblastic MC3T3-E1 cells were incubated in osteoblast differentiation medium containing CUR (10 μM), NAC (1000 μM), or DFO (100 μM) with or without FAC for additional 6 days and then washed with PBS, fixed with 4% paraformaldehyde for 30 min, and stained with 0.1% AR-S-Tris-HCl (pH 8.3) for 30 min at 37°C. Bone nodules were observed and photographed with a microscope. N: normal control group, cells were treated with DMSO; M: model group, cells were treated with FAC (500 μM); DFO: cells were treated with DFO (100 μM) and FAC (500 μM); NAC: cells were treated with NAC (1000 μM) and FAC (500 μM); CUR: cells were treated with CUR (10 μM) and FAC (500 μM).