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. 2019 Dec 24;2019:7838754. doi: 10.1155/2019/7838754

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Copyright © 2019 Yafan Gong et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) ROS generation was performed by immunofluorescence using DCFH-DA (green fluorescence, 5 mM) in cells. C indicates the control group; KD indicates the knockdown group. Cardiomyocytes were visualized using fluorescence microscopy. (b) The effects of IGF1 knockdown on the ROS levels in cardiomyocytes were detected by using a fluorescence microplate reader. C indicates the control group; KD indicates the knockdown group. ∗ shows a significant difference from the corresponding control (P < 0.05). n = 3.