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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Anticancer Res. 2009 Apr;29(4):1143–1150.

Figure 3.

Figure 3

Rapamycin effects on Akt, mTOR and its downstream targets in MCF10A cell lineage. Panel A: Immunoblot analysis of phospho-mTOR, phospho-Akt, phospho-4E-BP1, phospho-p70S6K (Thr389 and Thr421/Ser424), phospho-S6RP, and phospho-STAT3 (Ser727) protein levels following the treatment of each cell with 100 nM rapamycin for 1 and 24 h. Panel B: Scanning laser densitometry analysis of immunoblot band density. Rapamycin was least effective in inhibiting phospho-p70S6K and phospho-S6RP in the 10CA1a tumor cells.