Figure 4. NaCl-Primed or Normal iTregs Inhibit Differentiation of Pathogenic Cells in Inflammatory Colitis and Prevent Pathogenic Cells from Infiltrating to Colon.

(A) Colitis mice were sacrificed at 6 weeks after cell administration, and CD4⁺ cells from the spleen (SP) and mesenteric lymph nodes (mLN) were examined. Flow cytometric analysis and frequencies of IL-17A⁺CD90.2⁺ and IFNγ⁺CD90.2⁺ cells were examined in the respective mouse groups (6 mice in each group in one experiment).

(B) The bar graph depicts a summary of the experiment (6 mice in each group in one experiment).

(C) The infiltrating cells in large intestines (LP) were analyzed on total CD4⁺ T cells.

(D) The bar graph depicts a summary of the experiment (6 mice in each group in one experiment).

(E) On day 35 and 45 after cells transfer, iTreg⁻ Foxp3 loss were compared in SP and LN, cells were gated on CD90.1⁺. Representative results (mean ± SEM) from four independent experiments are shown.

(F) The line graph depicts a summary of the experiment (6 mice in each group in one experiment). Statistical analyses were performed using one-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.