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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Cell Rep. 2019 Feb 12;26(7):1869–1879.e3. doi: 10.1016/j.celrep.2019.01.066

Figure 6. High Salt Does Not Affect the Differentiation and Function of Human iTreg Cells.

Figure 6.

Human naive CD4+CD25−CD45RA+CD45RO− cells were stimulated with anti-CD3/28 microbeads in the presence of rhIL-2 and TGF-β in standard media, or in the presence of an additional 20 or 40 mM NaCl for 5 days.

(A) Foxp3 expression was detected by FACS.

(B) The bar graph depicts a summary of independent experiments in A (n = 5).

(C) T responder cells labeled with CFSE were stimulated with anti-CD3 in the presence of γ-irradiated antigen presenting cells (APCs). iTreg cells or iTregs primed with NaCl were added to culture wells. CFSE dilution and suppressive rates were calculated. Histograms depict cellular proliferation and are gated on CD8+ cells.

(D) The bar graph depicts a summary of independent experiments in (C) (n = 5). Statistical analyses were performed using one-way ANOVA. ns, not significant.