| Antibodies |
| Percp/cy5.5 anti-mouse CD4 antibody |
Biolegend |
Cat# 100540; RRID: AB_893326 |
| PE anti-mouse CD25 antibody |
Biolegend |
Cat# 101904; RRID: AB_312847 |
| APC anti-mouse CD62L antibody |
Biolegend |
Cat# 104412; RRID: AB_313099 |
| PE/Cy7 anti-mouse/human CD44 antibody |
Biolegend |
Cat# 103030; RRID: AB_830787 |
| LEAF Purified anti-mouse CD3ε antibody |
Biolegend |
Cat# 100331; RRID: AB_1877073 |
| PE anti-mouse IL17A antibody |
Biolegend |
Cat# 506904; RRID: AB_315464 |
| APC anti-mouse IFNγ antibody |
Biolegend |
Cat# 505810; RRID: AB_315404 |
| APC anti-mouse CD8a antibody |
Biolegend |
Cat# 100712; RRID: AB_312751 |
| GAPDH (D16H11) Rabbit mAb |
Cell Signaling Technology |
Cat# 5174; RRID: AB_10622025 |
| Phospho-FoxO1(Thr24)/FoxO3a(Thr32) Antibody |
Cell Signaling Technology |
Cat# 9464; RRID: AB_329842 |
| Anti-Rabbit IgG, HRP-linked Antibody |
Cell Signaling Technology |
Cat# 7074; RRID: AB_2099233 |
| Chemicals, Peptides, and Recombinant Proteins |
| Recombinant Human IL-2 Protein |
R&D |
Cat# 202-IL-050 |
| Recombinant Human TGF-β1 protein |
R&D |
Cat# 240-B |
| Sodium chloride solution |
Sigma Aldrich |
Cat# S5150 |
| Brefeldin A |
Sigma Aldrich |
Cat# B7651–5MG |
| Ionomycin calcium salt |
Sigma Aldrich |
Cat# I0634–1MG |
| Phorbol 12-myristate 13-acetate |
Sigma Aldrich |
Cat# P8139–1MG |
| Dynabeads Mouse T-activator CD3/28 |
Thermo Fisher |
Cat# 11453D |
| Dynabeads Human T-activator CD3/28 |
Thermo Fisher |
Cat# 11132D |
| X-VIVO15 |
LONZA |
Cat# 04–744Q |
| Critical Commercial Assays |
| CellTrace CFSE Cell proliferation kit |
Thermo Fisher |
Cat# C34554
|
| Naive CD4+ T cell isolation kit, mouse |
Miltenyi |
Cat# 130–104–453 |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6J |
The Jackson Laboratory |
Cat# 000664 |
| Mouse: B6-Foxp3 GFP
|
The Jackson Laboratory |
Cat# 006772 |
| Mouse: Rag1 KO |
The Jackson Laboratory |
Cat# 002216 |
| Software and Algorithms |
| FlowJo |
FlowJo, LLC |
https://www.flowjo.com/solutions/flowjo |
| GraphPad Prism |
GraphPad |
https://www.graphpad.com/ |
| Other |
| Sequence data, analyses. |
This paper |
N/A |
