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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Biochemistry. 2019 May 23;58(22):2617–2627. doi: 10.1021/acs.biochem.9b00303

Figure 2. Sequence logos for the fused 4-OT and Linker 2 clusters.

Figure 2.

A) The sequence logo for the fused 4-OT cluster. The sequences of the “fused” 4-OTs (86 total) are characterized by two 4-OT-like sections joined in the middle by a linker-region, which results in a gap in the multiple sequence alignments (MSA). The red asterisks indicate the conserved features (described in the text) and the red arrow indicates a conserved proline residue (red arrow logo-position 72) that is 10 positions before the arginine at logo-position 82. This could represent the position of an N-terminal proline before fusion of two short 4-OTs in many of the sequences. B) The sequence logo for the Linker 2 cluster. The sequences show the conserved features (described in the text), but the positioning is not as striking. Moreover, there is not an obvious conservation of proline as the start of the C-terminal section (red arrow logo-position 61). The logos are graphical representations of multiple sequence alignments (MSAs) generated using WebLogo.37