Figure 4.

A) Superimposition of fused 4-OT monomers, close-up to the linker loop region. B) Stick view of L65/P66 motif overlay of monomers A and B showing cis-proline configuration. C) Stick view of L65/P66 motif monomer C showing Pro-66 in the trans configuration. D) Sequence alignment of fused 4-OT, Linker 2, and the canonical 4-OT (labeled as F4-OT, L2, and canonical 4-OT). The secondary structure representation is labeled in the same way. Linker loop is enclosed in a box. A red asterisk shows Pro-1 of canonical 4-OT aligned to Pro-66 of fused 4-OT. The PRALINE sequence alignment tool was used to make this figure.⁴⁰