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Steady-state Kinetic Parameters for Fused 4-OT with Indicated Substrates.a
| Substrate |
kcat (s−1) |
Km (μM) |
kcat/Kmb (M−1 s−1) |
|---|---|---|---|
| 2-HM | 580 ± 25 | 208 ± 16 | 2.8 × 106 |
| 5-Me-2-HM | 72 ± 2 | 147 ± 9 | 4.9 × 105 |
| PP | 136 ± 8 | 720 ± 75 | 1.9 × 105 |
| HPD | 170 ± 12 | 1330 ± 140 | 1.3 × 105 |
| CHM | - | - | 2.3 × 103 |
The kinetic parameters were measured using the assay described in the text.
