Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic Parameters for the Fused 4-OT Mutants with 2-HM.a
| Enzyme | kcat (s−1) | rel. kcat | Km (μM) | rel. Km |
kcat/Km (M−1 s−1) |
rel. kcat/Km |
|---|---|---|---|---|---|---|
| WT | 580 ± 25 | 1.0 | 208 ± 16 | 1.0 | 2.79 × 106 | 1.0 |
| P1A | 3.4 ± 0.1 | 5.9 × 10−3 | 221 ± 11 | 1.06 | 1.54 × 104 | 5.5 × 10−3 |
| R39A | - | - | - | - | 8.34 × 103 | 3.0 × 10−3 |
| R76A | - | - | - | - | 3.74 × 103 | 1.3 × 10−3 |
| R104A | 313 ± 15 | 0.54 | 213 ± 18 | 1.02 | 1.47 × 106 | 0.53 |
| R127A | - | - | - | - | 2.84 × 104 | 1.0 × 10−2 |
The kinetic parameters were measured using the assay described in the text.
