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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Biochemistry. 2019 May 23;58(22):2617–2627. doi: 10.1021/acs.biochem.9b00303

Table 3.

Kinetic Parameters for the Fused 4-OT Mutants with 2-HM.a

Enzyme kcat (s−1) rel. kcat Km (μM) rel. Km kcat/Km
(M−1 s−1)
rel. kcat/Km
WT 580 ± 25 1.0 208 ± 16 1.0 2.79 × 106 1.0
P1A 3.4 ± 0.1 5.9 × 10−3 221 ± 11 1.06 1.54 × 104 5.5 × 10−3
R39A - - - - 8.34 × 103 3.0 × 10−3
R76A - - - - 3.74 × 103 1.3 × 10−3
R104A 313 ± 15 0.54 213 ± 18 1.02 1.47 × 106 0.53
R127A - - - - 2.84 × 104 1.0 × 10−2
a

The kinetic parameters were measured using the assay described in the text.

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