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Kinetic Analysis of Unfused 4-OT with 2-HM and PP.a
| Substrate |
kcat (s−1) |
Km (μM) |
kcat/Km (M−1 s−1) |
|---|---|---|---|
| 2-HM | 105 ± 6 | 148 ± 18 | 7.1 × 105 |
| PP | 19.3 ± 0.7 | 346 ± 28 | 5.6 × 104 |
The kinetic parameters were measured using the assay described in the text.
