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. 2019 Nov 1;4(21):e124644. doi: 10.1172/jci.insight.124644

Figure 2. Effect of CMRL 1066–based media and Transwell filters on the function of human islets during long-term culture.

Figure 2

(A–D) Fifty handpicked human islets from the same donor per replicate were cultured in a standard platform (96-well plate) (A and B) or Transwell filter (C and D) with different CMRL 1066–based media (CMRL, PIM, or CIT). Islet function in each replicate was assessed on day 1, 14, or 21 by perifusion. Results were obtained from 3 independent experiments, and each experiment was conducted in duplicate or triplicate with islets isolated from the same donor. The number of donors used in each condition (n = 2 or n = 3) is indicated. All data were pooled for each condition shown and plotted as mean ± SEM. All insulin values were normalized to DNA content of each sample. (E) Representative morphological assessment of long-term-cultured human islets. Human islets cultured in PIM or CIT medium remained viable but exhibited an aggregated morphology, with a darkening core reflecting central necrosis. Islets cultured in CMRL exhibited a loss of viability after 14-day culture. Scale bars: 100 μm.