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. 2019 Nov 1;4(21):e131152. doi: 10.1172/jci.insight.131152

Figure 5. Soluble Thy-1 reverses myofibroblastic differentiation of senescent human lung myofibroblasts in a dose-dependent manner in vitro.

Figure 5

Human lung fibroblasts (CCL-210) were incubated with human TGF-β1 (10 ng/ml for 48 hours) and subsequently subjected to 5-day serum-free media to induce senescence (n = 4/group, 3 times). (A) Myofibroblast differentiation and senescence were validated by qPCR of Acta2, Col1α1, and p21. (B) qPCR for Acta2 and Col1α1 after treatment of myofibroblasts with sThy-1-IgG Fc (10, 100, 1000 ng/mL), Thy-1(RLE)-IgG Fc (1000 ng/ml), or IgG Fc (1000 ng/ml) for 48 hours. (C) Western blot (WB) of αSMA expression. (D) WB quantification. Results are presented as mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test and 1-way ANOVA; *P < 0.05, **P < 0.01.