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. Author manuscript; available in PMC: 2020 Jan 8.
Published in final edited form as: Biotechnol J. 2015 Jul 14;10(10):1555–1567. doi: 10.1002/biot.201400756

Figure 1. μCP Well Plates.

Figure 1.

A) Workflow for creation of μCP Well Plates. B) Multi-well alignment device for μCP Well Plate assembly. Pictured: vacuum line (i), lift arm for vacuum tooling (ii), spring-leveled vacuum cup tooling (iii), micropatterned glass sheet (iv), 96-well plate with attached adhesive and cut-out well bottoms (v), and aluminum base with machined cavities designed to fit various μCP Well Plate components (vi). C) Cells on a 96-well μCP Well Plate. 6 wells of a fully patterned 96-well plate containing twelve circular features (300 μm radius) per well are shown. Human fibroblasts were seeded 72 hours prior to TRITC-phalloidin staining and imaging. Fluorescent signal at edge of wells is due to auto-fluorescence of tissue culture plastic at well edges, not cells at these locations. Inter-well areas are masked for clarity. D) Cells on 24-well μCP Well Plate. One well of a 24-well plate containing twelve 10×10 single-cell feature arrays per well is shown. Human fibroblasts were seeded 16 hours prior to TRITC-phalloidin staining and imaging.