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. 2019 Nov 14;4(22):e130835. doi: 10.1172/jci.insight.130835

Figure 1. NFKBIZ expression in mouse and human skin.

Figure 1

(A) Induction of IκBζ in whole-skin lysates from untreated and IMQ-treated, TAM-induced global (KO, upper) or keratinocyte-specific (K14-KO, lower) IκBζ-deficient mice at day 7. Actin served as a loading control. (B) Predominant localization of Nfkbiz in the epidermis of IMQ-treated control mice, which is absent in IMQ-treated K14-KO mice. Scale bars: 40 μm. (C) Keratinocyte-specific NFKBIZ expression was also detected in normal human skin (upper). As shown by the increased number of red dots, NFKBIZ expression was elevated in human psoriatic skin lesions (lower). Following deparaffinization tissue sections were hybridized with mouse or human NFKBIZ-specific RNAScope target probe sets consisting of multiple tandem short oligonucleotides. NFKBIZ mRNAs were visualized as dots, with each dot representing a single RNA transcript. Right images show sections of the pictures on the left at a higher magnification. Scale bars: 100 μm.