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. 2019 Nov 14;4(22):e130835. doi: 10.1172/jci.insight.130835

Figure 6. Deficiency of IκBζ in keratinocytes protects against IL-17A–dependent systemic inflammation.

Figure 6

(A) Gating strategy for the analysis of neutrophils and proinflammatory monocytes from blood and bone marrow. (B) Flow cytometry analysis of the numbers of circulating neutrophils (Ly6G+) and inflammatory monocytes (Ly6C+CCR2+) in the blood of IκBζ-proficient and -deficient K14-IL17Aind mice. n = 3–6 (mean ± SEM). (C) Relative levels of neutrophils (Ly6G+) and inflammatory monocytes (Ly6C+CCR2+) in the bone marrow of K14-IL17Aind mice as detected by flow cytometry. n = 3–5 (mean ± SEM). (D) Serum levels of G-CSF. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. n = 4 (mean ± SEM). P values were calculated using 2-tailed Student’s t test (*P < 0.05, **P < 0.01, and ***P < 0.001).