Figure 3. Dysregulated expression and stability of chaperones and heat shock genes/proteins in BAG3-R477H and BAG3-KO induced pluripotent stem cell–derived cardiomyocytes (iPSC-CMs).

(A) Principal components analysis of RNA-Seq gene expression profiles from unedited (WT), BAG3-R477H (RH), and BAG3-KO (KO) iPSC-CM. PC1 and PC2 denote principal components 1 and 2, respectively. (B) Relative transcript abundance of all chaperone and heat shock protein (HSP) genes in BAG3-RH vs. BAG3-WT iPSC-CMs (expressed as log₂ fold change). Red and gray points denote transcripts at P < 0.01 and FDR < 0.1 calculated by quasi-likelihood F-test (QLF test) in edgeR (28) and have been adjusted for multiple testing. (C) Western blot for BAG3, key binding partners, and GAPDH (loading control) in BAG3-WT (WT), BAG3-RH (RH), and BAG3-KO (KO) iPSC-CMs.