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. 2019 Nov 14;4(22):e126315. doi: 10.1172/jci.insight.126315

Figure 6. NF-κB signaling was activated in 4-week-old csPLA-Tg CMs and mediated by persistent DNA damage.

Figure 6

(A) Subcellular localization of p65 subunit of NF-κB in csPLA-Tg myocardium, highlighted by white arrows. (B) Quantification of p65 micrographs showing increases in the number of nuclei expressing p65 for 2- and 4-week-old hearts counted as a percentage of total nuclei. n = 3 females/group. Values are mean ± SD. *P < 0.05. Two-way ANOVA, no repeated measures, with Sidak’s test for multiple comparisons was performed. (C) Western blot showing increase of NF-κB subunit p65 in 4-week-old csPLA-Tg myocardium. (D) Confocal micrographs of fluorescence immunostaining showing DNA damage marker γ-H2AX (white arrows). Scale bar: 10 μm. (E) Quantification of γ-H2AX micrographs. n = 3 females/group. Values are mean ± SD. Two-way ANOVA, no repeated measures, with Sidak’s post hoc test for multiple comparisons was performed. (F) Western blots of 4-week-old myocardial lysates showing phosphorylation status of ATM and IκBα and graphs showing corresponding densitometry analyses. Values are mean ± SD. n = 3 females/group. Unpaired 2-tailed t test was performed. *P < 0.05, **P < 0.001.