Figure 3.
Overexpression of P97 affects photoreceptor survival. A, P97 protein level was detected in retinal lysates (2 μg total protein/lane) of one-month-old Gγ1-/- or P23H mice by Western blotting with an anti-P97 antibody; the densities of the P97 bands were normalized to the Hsc70 loading control. The number of mice analyzed was the following: WT, 6; Gγ1-/-, 6; P23H, 6. Each dot represents a single data point. B, P97 protein level was detected in retinal lysates (2 μg total protein/lane) from one-month-old WT and P97-overexpressing (P97oe) mice as in A; Hsc70 was used as a loading control. The number of mice analyzed was the following: WT, 5; P97oe, 5. Each dot represents a single data point. C, Spider diagrams representing the number of photoreceptor nuclei in 100-μm segments of the inferior and superior retina at various distances from the optic nerve head. The number of mice analyzed was the following: WT, 5; P97oe, 3. Mice were three months old. D, The total number of nuclei in all eight 100-μm retinal segments represented in spider diagrams of panel C. Each dot represents a single data point. E, Representative images of inferior and superior retina cross-sections from WT and P97oe at the 1-mm distance from the optic nerve head. Mice were three months old. Scale bar = 25 μm. Data are shown as mean ± SD. n.s. indicates p > 0.05. *p < 0.05, **p < 0.01.