Figure 1.

Delayed inhibition of sTNFα after T3Tx diminishes CGRP⁺ afferent plasticity near the central canal in lower lumbar cord. Representative images of transverse sections of L6 lumbar cord from T3Tx-Saline animals and T3Tx-XPro1595 animals 8 weeks post-injury and naive, uninjured animals immunostained for CGRP. a–d, T3Tx-Saline (b) and T3Tx-XPro1595 animals (c) had similar levels of CGRP⁺ expression in dorsal horn as naive, uninjured animals (a,d). e–h, There was increased CGRP⁺ immunoreactivity in T3Tx-Saline animals (f) in Laminae VII/X around the central canal compared with both naive animals (e) and T3Tx-XPro1595 animals (g,h). T3Tx-XPro1595 animals had normal levels of CGRP expression near the central canal. n = 3/4 per group. Mean ± SEM *p < 0.05. cc: central canal. Scale bar, 50 μm.