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. 2019 Sep 27;17(1):13–22. doi: 10.1080/15476286.2019.1669406

Figure 2.

Figure 2.

Heatmap of the percent activity value in each position. Darker grid indicates more frequent mismatch. The x-axis indicates nucleotide position while the y-axis shows all paired gRNA-DNA interactions with one nucleotide was removed from gRNA, producing a bulged DNA base.