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. 2019 Sep 16;17(1):33–46. doi: 10.1080/15476286.2019.1662268

Figure 1.

Figure 1.

CP affects cell proliferation and p27 mRNA stability in HEK293 but not in MCF7 cells. (a) Proliferation assay of cells treated (+CP; 20 µM) or not treated (-CP) with CP for the indicated time periods. (b) Trypan blue cell viability assay. Cells were counted (n = 3) and the result reported as a percentage of dead versus live cells in a bar plot. (c) Relative changes of p27 and p53 mRNAs in CP-treated (20 µM of CP for 15 h) compared to untreated (-CP) HEK293 and MCF7 cells as measured by RT-qPCR normalized to β-actin mRNA. Error bars represent the standard error of the mean (SEM), n = 3. *P < 0.05. (d) Immunoblot analysis with antibodies against the specified proteins. (e) HEK293 cells were treated with 20 µM CP for 15 h prior to the addition of 2 µg/ml of ActD for 30, 60, 90, 120, and 240 min. The half-life of p27 and c-myc mRNAs relative to β-actin was determined by RT-qPCR considering ‘one phase decay equation’ implemented in GraphPad Prism. Error bars represent SEM, n = 3.