Figure 4.

Knock-down of candidate RBPs affect p27 mRNA abundance upon CP treatment in HEK293 cells. (a) Cells were transiently transfected with siRNAs (siKHSRP, sihnRNPD, siPDAP1, siELAVL1, siSNW1) and Scr control oligos for 48 h and treated with 20 µM CP for the last 15 h. P27 mRNA levels of CP-treated (+CP) relative to untreated cells (-CP) was assessed by RT-qPCR and normalized to β-actin (left). P27 protein levels were quantified with immunoblots (right). Error bars represent SEM, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed student’s t-test.